Improvement of terrestrial groundwater sampling method affects microbial community analysis |
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Authors: | Akio Ueno Satoshi Tamazawa Shuji Tamamura Noritaka Aramaki A. K. M. Alam Badrul Takuma Murakami |
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Affiliation: | Horonobe Research Institute for the Subsurface Environment (H-RISE), Northern Advancement Centre for Science and Technology (NOASTEC), Teshio-gun, Hokkaido, Japan |
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Abstract: | Groundwater sampling is a critical step in subsurface microbial ecology. Here, we compared two different sampling methods: commonly used disposable bailers (unimproved sampler) and an improved sampler, the latter of which was devised to minimize exposure to the aerobic atmosphere. Microbial community analysis using the 16S rRNA and methyl coenzyme-M reductase (mcrA) genes in the lignite seam groundwater revealed that the archaeal communities in samples obtained by the improved sampler were dominated by hydrogenotrophic methanogen Methanobacterium. These results suggested that the improved sampler would be more favorable for obtaining methanogenic archaeal community than the unimproved one, and that the sampling method affected the microbial community analysis in the investigated subterranean lignite seams. |
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Keywords: | groundwater lignite mcrA gene subsurface microorganisms 16S rRNA gene |
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