Nocardioides rubroscoriae sp. nov., isolated from volcanic ash

A rod-shaped actinobacterium, designated Sco-A25^T, was isolated from a red-coloured layer of scoria (volcanic ash) in the Republic of Korea and subjected to a polyphasic taxonomic characterization. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain Sco-A25^T is a member of the genus Nocardioides and formed a tight monophyletic unit with the type strain of Nocardioides plantarum (98.7 % gene similarity). LL-Diaminopimelic acid was detected in the cell wall. The predominant menaquinone is MK-8(H₄). The polar lipids found were phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, and an unknown phospholipid. The fatty acid profile was represented by large amounts of saturated, unsaturated and iso-branched fatty acids. The DNA G+C content was 71.6 mol %. Genomic DNA similarity between strain Sco-A25^T and N. plantarum KCTC 9577^T was 47.8 %. On the basis of the results of phenotypic, genetic and phylogenetic analyses presented here, strain Sco-A25^T is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides rubroscoriae sp. nov. is proposed. The type strain is Sco-A25^T (=KCTC 19805^T = DSM 23986^T = NBRC 107916^T).