| 高效液相色谱法测定血浆中同型半胱氨酸 |
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| 引用本文： | 潘峰,孙玮,张青,罗婧,王丽婷,王芳,胡茂华,张正治. 高效液相色谱法测定血浆中同型半胱氨酸[J]. 氨基酸和生物资源, 2010, 32(4): 55-57. DOI: 10.3969/j.issn.1006-8376.2010.04.014 |
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| 作者姓名： | 潘峰 孙玮 张青 罗婧 王丽婷 王芳 胡茂华 张正治 |
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| 作者单位： | 第三军医大学,基础部,中心实验室,重庆,400038;第三军医大学,基础部,中心实验室,重庆,400038;第三军医大学,基础部,中心实验室,重庆,400038;第三军医大学,基础部,中心实验室,重庆,400038;第三军医大学,基础部,中心实验室,重庆,400038;第三军医大学,基础部,中心实验室,重庆,400038;第三军医大学,基础部,中心实验室,重庆,400038;第三军医大学,基础部,中心实验室,重庆,400038 |
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| 摘 要： | 建立了一种高效液相色谱-库仑阵列电化学测定血浆中同型半胱氨酸的方法。采用3-2-羧乙基膦作为还原剂还原巯基间形成的二硫键,用0.1 mol.L-1HC lO4来沉淀血浆蛋白,离心取上清,进样分析。在0.40~50.00μmol.L-1范围内,线性关系良好。加标平均回收率是98.43%,RSD%是4.35%。该方法简单、快速、灵敏。
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| 关 键 词： | 同型半胱氨酸 高效液相色谱法 库仑阵列电化学 血浆 |
Determination of Homocysteine in Plasma by High Performance Liquid Chromatography |
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| PAN Feng,ZHANG Qing,SUN Wei,LUO Jing,WANG Li-ting,WANG Fang,HU Mao-hua,ZHANG Zheng-zhi. Determination of Homocysteine in Plasma by High Performance Liquid Chromatography[J]. Amino Acids & Biotic Resources, 2010, 32(4): 55-57. DOI: 10.3969/j.issn.1006-8376.2010.04.014 |
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| Authors: | PAN Feng ZHANG Qing SUN Wei LUO Jing WANG Li-ting WANG Fang HU Mao-hua ZHANG Zheng-zhi |
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| Affiliation: | PAN Feng,ZHANG Qing,SUN Wei,LUO Jing,WANG Li-ting,WANG Fang,HU Mao-hua,ZHANG Zheng-zhi(Central Laboratory,College of Basic Medical Sciences,Third Military Medical University,Chongqing 400038,China) |
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| Abstract: | A high performance liquid chromatography(HPLC) with coularry electrochemical detection method for quantitative determination of human plasma homcysteine(Hcy) has been developed.Hcy was measured after the chemical reduction of all disulfide bonds with 100 μg· mL-1 tris(2-carboxyethyl)-phosphine hydrochloride(TCEP) and protein precipitation with 0.1 mol·L-1 HClO4.There was a good linear relationship between the content of Hcy and its peak area(r=0.999 8) in the range of 0.40-50.00μmol·L-1.The average recovery rate was 98.43% and the relative standard deviation(RSD) of intra-day was 4.35%.The method was simple,rapid and sensitive. |
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| Keywords: | homocysteine HPLC coularry electrochemical plasma |
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