Adjuvant properties of Cytosine-phosphate-guanosine oligodeoxynucleotide in combination with various polycations in an ovalbumin-vaccine model |
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Authors: | Maubant Sylvie Banissi Claire Beck Samantha Chauvat Anne Carpentier Antoine F |
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Institution: | Laboratoire de Recherches Biochirurgicales, H?pital Européen Georges Pompidou, Université Paris Descartes , Paris, France. |
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Abstract: | Oligonucleotides containing CpG motifs (cytosine-phosphate-guanosine oligodeoxynucleotide CpG ODN]) display strong immunostimulatory effects, and polycations have been previously reported as cellular delivery system. In the present study, we investigated the adjuvant properties of combinations of a CpG ODN with various polycations (poly-arginine, poly-lysine, poly-histidine, or chitosan) in an ovalbumin vaccination model. We showed that, when combined to CpG ODN, poly-arginine and poly-histidine, but not poly-lysine or chitosan, enhanced efficiently both the IgG antibody production and the number of splenocytes secreting interferon-gamma after stimulation with a CD8+ T cell-restricted peptide. Interestingly, CpG ODN-poly-arginine, which was the most efficient, compared favorably to the complete Freund's adjuvant and aluminium salts and induced no local toxicity, making this combination a very attractive adjuvant for vaccines. |
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