| HeLa细胞热休克蛋白的代谢动力学 |
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| 引用本文： | 徐明波,程素琦. HeLa细胞热休克蛋白的代谢动力学[J]. 中国生物化学与分子生物学报, 1990, 6(3): 268-272 |
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| 作者姓名： | 徐明波 程素琦 |
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| 作者单位： | 军事医学科学院卫生学环境医学研究所 天津(徐明波)，军事医学科学院卫生学环境医学研究所 天津(程素琦) |
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| 摘 要： | HeLa细胞经45℃,15min应激后,可诱导一组分子量为100,85,73,70,54kD的热休克蛋白,其中分子量为73/70kD的HSP产量最高。在产生HSP的同时,正常蛋白质合成受到抑制,并在随后数小时内恢复。HSP73/70在应激后能迅速诱导产生,应激后4—6小时为其合成高峰,10小时后明显减少,24小时恢复正常。其分解遵循指数规律,半衰期为49.9小时。
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| 关 键 词： | HeLa细胞 热休克蛋白 蛋白质生物合成 蛋白质代谢 |
| 收稿时间： | 1990-06-20 |
The Metabolic Dynamics of Heat Shock Protein in HeLa Cells |
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| Xu,Ming-bo Cheng,Su-qi. The Metabolic Dynamics of Heat Shock Protein in HeLa Cells[J]. Chinese Journal of Biochemistry and Molecular Biology, 1990, 6(3): 268-272 |
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| Authors: | Xu Ming-bo Cheng Su-qi |
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| Affiliation: | (Institute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences, Tianjin |
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| Abstract: | HeLa cells, when incubated at 45℃ for 15min, a group of heat shock proteins (HSP), with molecular weights of 100,85,73,70 and 54 kilodalton was synthesized. In these proteins, HSP73/70 had the highest yield. While HSP was produced, the rate of total protein synthesis underwent a rapid reduction, but recovered a few hours later. The rate of synthesis of the HSP73/70 reached a maximum value 4-6h after the heat shock, then gradually decreased to control value by 24hr. The rate of decay in HSP73/70 followed an exponential curve. The half-time for the degradation was 49.9hr. |
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| Keywords: | HeLs cells heat shock protein protein synthesis protein metabolism |
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