Generalized recombination in tandem duplications of bacteriophage lambda |
| |
Authors: | John B. Hays and Robert J. Zagursky |
| |
Affiliation: | (1) Department of Chemistry, University of Maryland, Baltimore County, 21228 Catonsville, Maryland, USA |
| |
Abstract: | Summary Recombination between the tandem duplicated segments of b221a106-15 yields unduplicated (single-copy) b221 phage. The apparent frequency of intramolecular events among these recombinations was determined for both cellular (Rec) and bacteriophage (Red) generalized recombination systems. The progeny from single-cycle growth experiments with genetically marked duplication phages were treated with EDTA to inactivate all but the singlecopy phages produced by recombination. Analysis of the genotypes of the EDTA-resistant phages suggested that intramolecular events were about 1 to 5 times as frequent as intermolecular ones. While the results suggest that intramolecular events are not intrinsically forbidden, the quantitative values for the ratio depend on the assumption that intracellular phage chromosomes are completely mixed. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|