Production of lipocortin-like proteins by cultured human tracheal submucosal gland cells |
| |
Authors: | J Jacquot F Dupuit H Elbtaouri J Hinnrasky F Antonicelli B Haye E Puchelle |
| |
Institution: | INSERM U314, Université de Reims, CHU Maison Blanche, France. |
| |
Abstract: | Evidence is obtained for the presence of lipocortin-like proteins in human tracheal gland cells in culture. Using polyclonal antibodies to lipocortin I, indirect immunofluorescence studies demonstrate that lipocortin I is mainly confined to the tracheal gland cell surface. From cell membranes, four Ca2(+)-dependent proteins (35, 40, 45 and 67 kDa) were identified as lipocortin related proteins by using immunoblotting and fluorography following 35S]methionine metabolic labeling experiments. A strong immunoreactivity for the 35 kDa protein was observed. In addition, lipocortin-like proteins with apparent Mr33, 35, 37 and 67 kDa, respectively, were released in the apical culture medium by tracheal gland cells cultured on microporous membrane of a double chamber culture system. |
| |
Keywords: | |
|
|