Fine structure and assembly in vitro of nuclear lamina in plant cells |
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| Authors: | WANG JianYANG ChengHANS RisZHAI Zhonghe |
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| Institution: | (1) College of Life Sciences, Peking University, 100871 Beijing, China;(2) Integrated Microscopy Resource, University of Wisconsin, Madison, USA;(3) Present address: Department of Biochemistry, Max Planck Institute for Biophysical Chemistry, PO Box 2841, D-37018 Goettingen, Germany |
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| Abstract: | The fine structure of the nuclear lamina (NL) in sperm cells ofGinkgo biloba was visualised using high resolution low-voltage scanning electron microscopy (LVSEM). It was shown that the nuclear lamina
was composed of 10 nm filaments which formed a fine network. Lamins were purified from cultured carrot suspension cells and
assembledin vitro. Long 8–12 nm diameter filaments were seen and sometimes subfilaments could be distinguished. Western blot of filament preparations
showed that these contained the 66 and 84 ku lamins. These data demonstrate that plant lamins are capable of assembling into
filamentsin vitro.
Project supported by the National Natural Science Foundation of China (Grant No. 3500073). |
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| Keywords: | plant cells nuclear lamina ultrastructure assembly in vitro |
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