Multiple-flash activation of the water-photolysis system in wheat leaves as observed by delayed emission |
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Authors: | Yorinao Inoue |
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Institution: | Laboratory of Plant Physiology, The Institute of Physical and Chemical Research (Rikagaku Kenkyusho), Wako-shi, Saitama, Japan |
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Abstract: | The chloroplasts from wheat leaves greened under intermittent illuminations (1 ms in duration) at long intervals (5 min) are capable of photoreducing DCIP (2, 6-dichlorophenolindophenol) with diphenylcarbazide as an electron donor but are incapable of photoreducing DCIP with water as the donor. On exposure of such intermittently illuminated leaves to flashes spaced at intervals of less than 10 s, the delayed light emission from the leaves was greatly enhanced in parallel with the generation of Hill activity. The mechanism of this photoactivation was studied by following the changes of the delayed emission from intermittently illuminated leaves exposed to short-interval flashes programmed in various ways. Analysis of the kinetic data indicated that the photoactivation involves three consecutive photoreactions with a rate-limiting dark reaction between them; P-light → A0-light → A1-dark → A2-light → A3 in which P is a precursor convertible to A0, the first intermediate with a longer lifetime of and A3 is the final activated compound or state converted by short-interval flashes from A0 through A1 and A2, two other intermediates with shorter lifetimes of and 5 s, respectively. |
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Keywords: | DCIP 2 6-dichlorophenolindophenol dark interval between short-interval flashes in an assemblage dark interval between multiple-flash assemblages number of short-interval flashes in an assemblage number of multiple-flash assemblages given to intermittently illuminated leaves delayed emission measured as total photon count |
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