Short communication: Construction of a cloning vector for Paenibacillus polymyxa and P. azotofixans |
| |
| Authors: | G. F. Duarte L. Seldin |
| |
| Affiliation: | (1) Departamento de Microbiologia Geral, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco I, IIha do Fundão, CEP 21941-590 Rio de Janeiro, Brazil |
| |
| Abstract: | A cloning vector that could replicate in Paenibacillus polymyxa, P. azotofixans and Bacillus subtilis was constructed using two Staphylococcus aureus plasmids. The recombinant plasmid confers chloramphenicol and erythromycin resistance and contains unique restriction sites for PvuII and BclI. The stability of pRJ45 was analysed. |
| |
| Keywords: | Cloning vector Paenibacillus polymyxa Paenibacillus azotofixans |
| 本文献已被 SpringerLink 等数据库收录！ |
|
