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Quantitative proteome analysis using D-labeled N-ethylmaleimide and 13C-labeled iodoacetanilide by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
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| Authors: | Kurono Sadamu Kurono Tamie Komori Naoka Niwayama Satomi Matsumoto Hiroyuki |
| Institution: | aDepartment of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA bDepartment of Chemistry, Oklahoma State University, Stillwater, OK 74078, USA cDepartment of Chemistry and Biochemistry, Texas Tech University, Box 41061, Lubbock, TX 79409-1061, USA dIBERICA HOLDINGS Co., Ltd, Kurume University Translational Research Center, Kurume, Fukuoka 830-0011, Japan |
| Abstract: | A new methodology for quantitative analysis of proteins is described, applying stable-isotope labeling by small organic molecules combined with one- or two-dimensional electrophoresis and MALDI-TOF-MS, also allowing concurrent protein identification by peptide mass fingerprinting. Our method eliminates fundamental problems in other existing isotope-tagging methods requiring liquid chromatography and MS/MS, such as isotope effects, fragmentation, and solubility. It is also anticipated to be more practical and accessible than those LC-dependent methods. |
| Keywords: | Quantitative analysis Proteomics Stable-isotope labeling Cysteine modifier |
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