Micropropagation of the silk tassel bush,Garrya elliptica Dougl. |
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| Authors: | Stephen Woodward Robert J. Thomson |
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| Affiliation: | (1) Oxford Forestry Institute, University of Oxford, Wytham, OX2 8QT Oxford, UK;(2) Department of Plant Sciences, Micropropagation Laboratory, University of Oxford Field Laboratory, Wytham, OX2 8QT Oxford, UK;(3) Present address: Department of Forestry, University of Aberdeen, St. Machar Drive, AB9 2UD Aberdeen, Scotland;(4) Present address: Department of Agriculture, Perth, Western Australia |
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| Abstract: | Proliferating cultures ofGarrya elliptica were established from shoot tip and nodal explants grown on woody plant medium containing benzyladenine. Optimum numbers of healthy shoots were induced on media containing 1.25  M benzyladenine; higher concentrations caused more hyperhydricity and less extension. Following growth for 28 days on quarter-strength woody plant medium containing 0.2% activated charcoal, 90% of shoots rooted on the same medium lacking charcoal, but with 2.5 M indolebutyric acid. Survival during weaning was poor, and was strongly correlated with the time of year at which plants were removed from culture.Abbreviations WPM McCown & Lloyd's (1980) Woody Plant medium - BA Benzyladenine - IAA Indoleacetic acid - IBA Indolebutyric acid - NAA Naphthaleneacetic acid |
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| Keywords: | Garrya elliptica micropropagation shoot quality weaning |
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