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Bioengineering and characterization of DNA-protein assemblies floating on supported membranes.
Authors:Wilfrid Boireau  Sophie Bombard  Marie-Agnès Sari  Denis Pompon
Institution:Laboratoire d'lngénierie des Protéines Membranaires, Centre de Génétique Moléculaire, CNRS, F-91198 Gif-sur-Yvette Cedex, France.
Abstract:A biodevice involving thiolated ssDNA and engineered cytochrome b5 linked through a cis-platine bridge is described. This original nanostructure is associated to a supported membrane through a floating anchor, thus constituting a dynamic bidimensionnal DNA capture device contrasting with the constrained geometry of currently available DNA chips. Characterization by optical spectroscopy, surface plasmon resonance and gel electrophoresis demonstrated that an unique molecular supra-assembly featuring specific DNA recognition capability has been obtained. This device is characterized by the reversibility of its assembly, self-organization and fluidity properties and is of interest as a prototype to design new generations of DNA chip biosensors.
Keywords:biosensor  hybrid bilayer  lateral mobility  redox protein  DNA chips  nanotechnology  plasmon resonance
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