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Characterization of Pseudomonas aeruginosa phage KPP21 belonging to family Podoviridae genus N4‐like viruses isolated in Japan |
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| Authors: | Ryu Shigehisa Jumpei Uchiyama Shin‐ichiro Kato Iyo Takemura‐Uchiyama Kotoe Yamaguchi Reina Miyata Takako Ujihara Yoshihiko Sakaguchi Noriaki Okamoto Hidekatsu Shimakura Masanori Daibata Masahiro Sakaguchi Shigenobu Matsuzaki |
| Institution: | 1. Department of Microbiology and Infection, Kochi Medical School, Kochi University, Kochi;2. Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, Kanagawa;3. Science Research Center, Kochi University, Kochi;4. Department of Microbiology, Kitasato University School of Medicine, Kanagawa, Japan |
| Abstract: | Bacteriophages (phages) belonging to the family Podoviridae genus N4‐like viruses have been used as therapeutic agent in phage therapy against Pseudomonas aeruginosa infections. P. aeruginosa phage KPP21 was isolated in Japan, and phylogenetically investigated the phages belonging to this viral genus. Morphological and genetic analyses confirmed that phage KPP21 belongs to the family Podoviridae genus N4‐like viruses. Moreover, phylogenetic analyses based on putative DNA polymerase and major virion protein showed that P. aeruginosa phages belonging to the genus N4‐like viruses are separated into two lineages and that phage KPP21 is in the same clade as phage LUZ7. |
| Keywords: | bacteriophage phage therapy phylogeny Pseudomonas aeruginosa |