Synthetic p55 tandem DNA vaccine against Pneumocystis carinii in rats |
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Authors: | Hua Fan Jiu‐Ying Guo Su‐Li Ma Nan Zhang Chun‐Li An |
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Institution: | Department of Pathogen Biology, College of Basic Medical Sciences, China Medical University, Shenyang 110122, China |
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Abstract: | Pneumocystis spp. are opportunistic fungal pathogens that are closely associated with severe pneumonia and pulmonary complications in patients with impaired immunity. In this study, the antigenic epitopes of the gene encoding the 55 kDa antigen fragment of Pneumocystis (p55), which may play an important role in Pneumocystis pneumonia, were analyzed. A gene containing tandem variants of the p55 antigen was synthesized and named the tandem antigen gene (TAG). TAG's potential as a DNA vaccine was assessed in immunosuppressed rats. Immunization with p55‐TAG DNA vaccine significantly reduced both the pathogen burden and lung–weight to body–weight ratios. Additionally, p55‐TAG vaccination in immunosuppressed rats elicited both cell‐mediated and humoral immunity. |
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Keywords: | Pneumocystis p55 DNA vaccine tandem antigen gene |
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