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Isolation and characterization of microsatellite loci from Corydalis ambigua (Papaveraceae) |
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| Authors: | Ikumi Dohzono Shingo Kaneko Jun Yokoyama Yuji Isagi |
| Affiliation: | 1. Graduate School of Human Development and Environment, Kobe University, Kobe, Japan;2. Graduate School of Agriculture, Kyoto University, Kyoto, Japan;3. Graduate School of Symbiotic Systems Science and Technology, Fukushima University, Fukushima, Japan;4. Department of Biology, Faculty of Science, Yamagata University, Yamagata, Japan |
| Abstract: | Primers for seven unlinked and highly polymorphic microsatellite loci were developed for Corydalis ambigua to investigate its mating system and population genetics. This species can be used to explore the impact of an alien bumblebee on plant reproduction. Genetic diversity and other population genetic parameters were estimated in two populations with and without the alien bumblebee. The number of alleles per locus ranged from 4 to 20, and observed heterozygosities ranged from 0.355 to 0.969. These markers can be applied to study mating systems and population genetics in C. ambigua. |
| Keywords: | Allele frequency bumblebee pollination Corydalis ambigua microsatellite loci polymorphism |