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Impaired Japanese encephalitis virus replication in p62/SQSTM1 deficient mouse embryonic fibroblasts |
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| Authors: | Takafumi Tasaki Souichi Nukuzuma Tsutomu Takegami |
| Institution: | 1. Division of Protein Regulation Research, Department of Life Science, Medical Research Institute, Kanazawa Medical University, 1‐1 Daigaku, Uchinada, Kahoku‐gun, Ishikawa 920‐0293, Japan;2. Department of Infectious Diseases, Kobe Institute of Health, Chuo‐ku, Kobe 650‐0046, Japan;3. Department of Life Science, Medical Research Institute, Kanazawa Medical University, Kahoku‐gun, Ishikawa 920‐0293, Japan |
| Abstract: | The role of the autophagy adaptor protein p62/SQSTM1 in Japanese encephalitis virus (JEV) replication in mouse embryonic fibroblasts (MEFs) was investigated. Amounts of JEV RNA and E protein were significantly smaller in p62‐deficient cells than wild‐type cells at 24 hr post‐infection (p.i.). JEV RNA quantitation and viral plaque assays showed significant reductions in viral titers in p62‐deficient cell culture fluid. Our results indicate that JEV replication is impaired in p62‐deficient MEFs, suggesting that p62 positively regulates JEV replication in host cells. |
| Keywords: | autophagy JEV p62/SQSTM1 |