Efficient isolation of human parainfluenza viruses 1 and 3 using MNT‐1, a human malignant melanoma cell line system that exhibits an apparent cytopathic effect |
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| Authors: | Ko Sato Oshi Watanabe Suguru Ohmiya Fumiko Chiba Masahiro Hayashi Tamio Suzuki Kazuyoshi Kawakami Hidekazu Nishimura |
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| Affiliation: | 1. Virus Research Center, Clinical Research Division, Sendai Medical Center, 2‐8‐8 Miyagino, Miyagino‐ku, Sendai 983‐8520, Japan;2. Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, 2‐1 Seiryo‐machi, Aoba‐ku, Sendai 980‐8575, Japan;3. Department of Dermatology, Faculty of Medicine, Yamagata University, 2‐2‐2 Iida‐nishi, Yamagata 990‐9985, Japan |
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| Abstract: | Isolation of human parainfluenza virus (HPIV) serotypes 1 and 3 from clinical specimens is not very efficient because of the lack of a cell culture system capable of inducing CPE. In this study, the utility of a melanoma cell line, MNT‐1, that allows HPIV growth and displays CPE was demonstrated. In particularly, the efficiency of isolating HPIV1 and HPIV3 using MNT‐1 was greater than for cell lines conventionally used for HPIV isolation. Our demonstrated efficacy of HPIV1 and HPIV3 isolation with apparent CPE using the MNT‐1 cell culture system has the potential to improve virus isolation from clinical specimens. |
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| Keywords: | cytopathic effect human parainfluenza virus MNT‐1 cells virus isolation |
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