Isolation and characterization of lysosomal alpha-mannosidase of placental tissue |
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Authors: | Farhat A Khan Debkumar Basu |
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Institution: | (1) Neurochemistry Division, Sree Chitra Tirunal Institute for Medical Sciences and Technology, 695 011 Trivandrum |
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Abstract: | The acidic α-mannosidase was purified 4400-fold by affinity chromatography on Concavalin A-Sepharose and heat treatment at
65‡C in the presence of 1 mM zinc ion. The enzyme did not resolve into multiple forms as in the case of enzymes from human
liver and human kidney. The pH optimum of the enzyme was 4.2 in citrate-phosphate buffer. The Km value for p-nitrophenyl-α-D-mannose was 1.9 mM. The molecular weight of the enzyme determined by gel filtration was 300,000.
The enzyme contained 10.6% neutral sugars. |
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Keywords: | Placental tissue acid α -mannosidase Concanavalin A-Sepharose chromatography |
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