Propagation of mouse mammary tumor cell lines and production of mouse mammary tumor virus in a serum-free medium |
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Authors: | Richard F Bauer Larry O Arthur Donald L Fine |
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Institution: | (1) NCI Frederick Cancer Research Center, P. O. Box B, 21701 Frederick, Maryland |
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Abstract: | Summary Five different mouse mammary tumor cell lines were propagated in a serum free medium. Evaluation of growth characteristics,
including logarithmic growth, cell population increase, protein production and days to confluency, showed serum-free medium
comparable to serum-containing medium. Mouse mammary tumor virus expression and production, in C3H and GR tumor cell lines, as determined by virus particle counting and RNA dependent DNA polymerase assays, subsequent to
dexamethasone stimulation revealed equivalent to higher levels of virus in serum-free medium as compared to serum-containing
medium. |
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Keywords: | serum-free mouse mammary tumor virus mouse mammary tumor cells dexamethasone |
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