Determination of captopril in human blood by high-performance liquid chromatography with electrochemical detection |
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| Authors: | Kazutake Shimada Makoto Tanaka Toshio Nambara Yutaka Imai Keishi Abe Kaoru Yoshinaga |
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| Institution: | Pharmaceutical Institute, Tohoku University, Sendai 980 Japan;The Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai 980 Japan |
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| Abstract: | A new method for quantitation of captopril in human blood is described. Captopril was derivatized with N-(4-dimethylaminophenyl)maleimide into the electrochemically active adduct. The derivative was separated and determined by high-performance liquid chromatography with an electrochemical detector on a reversed-phase column. The proposed method was satisfactory for determination of captopril in whole blood with respect to accuracy and precision. The detection limit of captopril thereby obtained was 10 ng/ml. The blood levels of captopril in patients orally given an officinal dose were measured by the present method. |
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