Knock-Down of the 37-kDa/67-kDa Laminin Receptor in Mouse Brain by Transgenic Expression of Specific Antisense LRP RNA |
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| Authors: | Leucht Christoph Vana Karen Renner-Müller Ingrid Dormont Dominique Lasmézas Corinne Ida Wolf Eckhard Weiss Stefan |
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| Affiliation: | Laboratorium für Molekulare Biologie Genzentrum Institut für Biochemie der LMU München, Feodor-Lynen-Strasse 25, D-81377 Munich, Germany. |
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| Abstract: | The 37-kDa/67-kDa laminin receptor (LRP/LR) plays a major role in the propagation of PrPSc, the abnormal form of the prion protein. In order to ablate the expression of LRP/LR in mouse brain we generated transgenic mice ectopically expressing antisense LRP RNA in the brain under control of the neuron-specific enolase (NSE) promoter. Hemizygous transgenic mice TgN(NSEasLRP)2 showed a significant reduction of LRP/LR protein levels in hippocampal and cerebellar brain regions. These mice might act as powerful tools to investigate the role of the laminin receptor in scrapie pathogenesis. |
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| Keywords: | antisense RNA laminin receptor LRP/LR neuron-specific enolase (NSE) prion PrP |
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