A Cyclic Nucleotide-dependent Chloride Conductance in Olfactory Receptor Neurons |
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| Authors: | RJ Delay AE Dubin VE Dionne |
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| Institution: | (1) Boston University Marine Program, Marine Biological Laboratory, Woods Hole, MA 02543, USA, US;(2) Department of Biology, San Diego State University, San Diego, CA 92182, USA, US;(3) Department of Medicine, University of California San Diego, La Jolla, CA 92093, USA, US |
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| Abstract: | Whole-cell membrane currents were recorded from olfactory receptor neurons from the neotenic salamander Necturus maculosus. Cyclic nucleotides, released intracellularly by flash photolysis of NPE-caged cAMP or NPE-caged cGMP, activated a transient
chloride current. The chloride current could be elicited at constant voltage in the absence of extracellular Ca2+ as well as in the presence of 3 mm intracellular Ca2+, suggesting that the current did not require either voltage or Ca2+ transients for activation. The current could be elicited in the presence of the protein kinase inhibitors H-7 and H-89, and
in the absence of intracellular ATP, indicating that activation was independent of protein kinase A activity. These results
suggest that Necturus olfactory receptor neurons contain a novel chloride ion channel that may be directly gated by cyclic nucleotides.
Received: 12 November 1996/Revised: 4 April 1997 |
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| Keywords: | : Chloride conductance — Cyclic nucleotide — Channel regulation — Cyclic AMP |
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