The Effect of Divalent Cations and Core Polymerase in the Abortive Initiation by Escherichia Coli Dna-Dependent Rna Polymerase Using Phosphonate Dinucleotide Primers |
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Authors: | Ales Cvekl Kv ta Horsk Karel ebesta Ivan Rosenberg Antoní n Holý |
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Affiliation: | a Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague 6, Czechoslovakia |
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Abstract: | The effect of divalent Mg2+ and Mn2+ cations on the elongation of ApU, UpA and their 3'-O- and 5'-O-phosphonylmethyl analogues by RNA polymerase holoenzyme to the corresponding trinucleo-tides on a poly(dA-dT) template was investigated. In contrast to Mgz+ ions, Mn2+ ions enhance abortive trinucleotide synthesis. This effect is more pronounced with phosphonylmethyl analogues. The core enzyme cannot catalyze the elongation of either (2'-5') UpA or phosphonylmethyl analogues. The localization of the divalent cation activator, as well as the role of the σ subunit at the catalytic centre of the holoenzyme, is discussed. |
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Keywords: | Divalent cations Core polymerase Phosphonate dinucleotide primers |
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