Species differences in localization of cardiac cAMP-phosphodiesterase activity: A cytochemical study |
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Authors: | L'udmila Okruhlicová Narcisa Tribulová Ján Styk Anita Eckly Claire Lugnier Ján Slezák |
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Affiliation: | (1) Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Repahlic;(2) Laboratoire de Pharmacologie et Physiologie Cellulaires-CNRS, Université L. Pasteur de Strasbourg, Illkirch, France |
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Abstract: | The localization of the membrane-bound cyclic 3 ,5 -AMP phosphodiesterasein cardiac tissues of both, rat and dog was studied by cytochemical method.40 µm thick slices from glutaraldehyde fixed heart tissue wereincubated in the medium with cAMP as a substrate and Pb ions as a capturemetal of the reaction product. The cAMP-PDE activity in the rat ventriclewas only shown positive on the sarcolemma. Whereas, in canine ventriculartissue the cAMP-PDE activity in cardiomyocytes was shown on the sarcolemma,on the junctional sarcoplasmic reticulum and on subsarcolemmal cisternae.The results confirm differences in the localization of cAMP-PDE in dog andrat heart. |
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Keywords: | phosphodiesterase cytochemistry myocardium rat dog |
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