Immunogenicity of the spike glycoprotein of Bat SARS-like coronavirus |
| |
Authors: | Yu-xuan Hou Cheng Peng Zheng-gang Han Peng Zhou Ji-guo Chen Zheng-li Shi |
| |
Institution: | 1.State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China2.Department of Biological Sciences, Mississippi State University, Mississippi State, MS 39762, USA |
| |
Abstract: | A group of SARS-like coronaviruses (SL-CoV) have been identified in horseshoe bats. Despite SL-CoVs and SARS-CoV share identical
genome structure and high-level sequence similarity, SL-CoV does not bind to the same cellular receptor as for SARS-CoV and
the N-terminus of the S proteins only share 64% amino acid identity, suggesting there are fundamental differences between
these two groups of coronaviruses. To gain insight into the basis of this difference, we established a recombinant adenovirus
system expressing the S protein from SL-CoV (rAd-Rp3-S) to investigate its immune characterization. Our results showed that
immunized mice generated strong humoral immune responses against the SL-CoV S protein. Moreover, a strong cellular immune
response demonstrated by elevated IFN-γ and IL-6 levels was also observed in these mice. However, the induced antibody from
these mice had weaker cross-reaction with the SARS-CoV S protein, and did not neutralize HIV pseudotyped with SARS-CoV S protein.
These results demonstrated that the immunogenicity of the SL-CoV S protein is distinct from that of SARS-CoV, which may cause
the immunological differences between human SARS-CoV and bat SL-CoV. Furthermore, the recombinant virus could serve as a potential
vaccine candidate against bat SL-CoV infection. |
| |
Keywords: | SARS coronavirus(SARS-CoV) SARS-like coronavirus(SL-CoV) Spike glycoprotein Humoral immune response Cellular immune response |
本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录! |
| 点击此处可从《中国病毒学》浏览原始摘要信息 |
| 点击此处可从《中国病毒学》下载免费的PDF全文 |
|