2D-DIGE analysis of mango (Mangifera indica L.) fruit reveals major proteomic changes associated with ripening |
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Authors: | Andrade Jonathan de Magalhães Toledo Tatiana Torres Nogueira Silvia Beserra Cordenunsi Beatriz Rosana Lajolo Franco Maria do Nascimento João Roberto Oliveira |
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Institution: | 1. University of São Paulo, Department of Food Science and Experimental Nutrition, FCF, São Paulo, Brazil;2. University of São Paulo – NAPAN – Food and Nutrition Research Center, Brazil |
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Abstract: | A comparative proteomic investigation between the pre-climacteric and climacteric mango fruits (cv. Keitt) was performed to identify protein species with variable abundance during ripening. Proteins were phenol-extracted from fruits, cyanine-dye-labeled, and separated on 2D gels at pH 4-7. Total spot count of about 373 proteins spots was detected in each gel and forty-seven were consistently different between pre-climacteric and climacteric fruits and were subjected to LC-MS/MS analysis. Functional classification revealed that protein species involved in carbon fixation and hormone biosynthesis decreased during ripening, whereas those related to catabolism and the stress-response, including oxidative stress and abiotic and pathogen defense factors, accumulated. In relation to fruit quality, protein species putatively involved in color development and pulp softening were also identified. This study on mango proteomics provides an overview of the biological processes that occur during ripening. |
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Keywords: | Mango Ripening Postharvest Proteomics Fruit quality |
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