Regeneration of comparative genomic hybridization oligonucleotide microarrays with dimethylurea |
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Authors: | Ronowicz Anna Brzeskwiniewicz Magdalena Madanecki Piotr Buckley Patrick G Orłowska Ewa Renata Ochocka J Limon Janusz Piotrowski Arkadiusz |
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Institution: | Department of Biology and Pharmaceutical Botany, Medical University of Gdansk, 80-416 Gdansk, Poland. |
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Abstract: | Reuse of materials in DNA hybridization-based methods has been known since the advent of Southern membranes. Array-based comparative genomic hybridization is essentially Southern hybridization with multiple probes immobilized on a solid surface. We show that comparative genomic hybridization microarrays fabricated with maskless array synthesizer technology can be used up to four times with the application of 1,3-dimethylurea as an array-stripping agent. We reproducibly detected chromosomal aberrations (0.6-22.4Mb in size) in four hybridization rounds using regenerated microarray slides. We also demonstrated that regenerated arrays can detect smaller alterations (16-200kbp), such as common copy number variants, as well as complex aberration profiles in tumor DNA. |
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