Asialoglycoprotein receptor and liposome synergistically mediate the gene transfer into primary rat hepatocytes |
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Authors: | LI Chonghui WEN Shouming ZHAI Haifeng SUN Manji |
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Affiliation: | (1) Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, 100850 Beijing, China;(2) Department of Clinic Pharmacology, General Hospital of Air Force, 100036 Beijing, China;(3) Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, 100850 Beijing, China |
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Abstract: | Gene transfer into primary rat hepatocytes was performed by employing cationic liposome as DNA carrier and the specific ligand of hepatic asialoglycoprotein receptor (ASGPR), asialofetuin, as liver-targeting ligand. The results showed that asialofetuin, when added to the gene transfer complexes, could significantly increase the hepatocyte transfection efficiency, and alleviate the cellular toxicity of Lipofectin. Several synthetic ligands of ASGPR (galactosyl albumin) could also increase the transfection efficiency of hepatocyte like asialofetuin. It was proved that ASGPR and cationic liposome could synergistically mediate the gene transfer into primary rat hepatocytes. This novel gene delivery system provided a safer, more simple and efficient gene transfer method for primary hepatocytes, and showed prospecting application in hepatic gene therapy. |
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Keywords: | asialoglycoprotein receptor cationic liposome receptor-mediated gene transfer primary hepatocyte. |
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