Coumarin effects on Glycine max hypocotyl explants |
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Authors: | EVA JANSSON SVEN-BÖRJE SVENSSON |
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Institution: | Department of Plant Physiology, University of Lund, Box 7007, S-220 07 Lund, Sweden |
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Abstract: | Coumarin induced root formation and stimulated fresh weight production in hypocotyl explants of Glycine max L. cultured in vitro. All stimulatory effects caused by coumarin were induced within a relatively narrow range of concentrations between 1–500 μM, yielding optimum dose response curves. When coumarin was combined with kinetin fresh weight increased considerably, at optimum concentrations to a level almost as high as that obtained with NAA (10 μM) and kinetin (10 μM). Root formation was almost completely inhibited when kinetin was added in combination with coumarin. NAA + coumarin had small stimulatory effects on fresh weight. but were inhibitory in root formation. The frequency of rooting per explant, texture and pigmentation were also affected by different treatments. |
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Keywords: | Coumarin Glycine max soybean root formation in vitro culture |
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