Rhizobial siderophore as an iron source for clover |
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Authors: | Mieczyslawa Derylo Anna Skorupska |
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Affiliation: | Inst. of Microbiology, Univ. M. Curie Sklodowska, 19 Akademicka Str. P-20-033 Lubin. Poland. |
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Abstract: | Iron uptake by clover plants ( Trifoliuin pratense L. cv. Hruszowska) was studied using radioactive ferric iron (55FeCl2). As shown by autoradiography of non-infected plants, purified rhizobial siderophore isolated from Rhizobium leguminosarum by, trifolii , stimulated the uptake and shoot transport of iron. Addition of rhizobial siderophore into the growth medium of nodulated clover did not affect the iron transport to the shoots. In the absence of the rhizobial siderophore, clover infected by either nitrogen-fixing (Nod− Fix+) or nonfixing (Nod+ Fix−) R. L. trifolii strains took up and transported into the shoots more iron than the non-infected control plants. Nodulated clover reduced Fe(III) more efficiently than the non-infected control plants. |
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Keywords: | Clover iron uptake nitrogen fixatior nodulation Rhizobium leguminosarum bv irifolii siderophore Trifolium pratense |
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