Cloning of the Escherichia coli K-12 hemB gene. |
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| Authors: | J M Li H Umanoff R Proenca C S Russell S D Cosloy |
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| Affiliation: | Department of Biochemistry, City College, City University of New York, New York 10031. |
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| Abstract: | An Escherichia coli heme-requiring, heme-permeable mutant had no detectable 5-aminolevulinate dehydratase or porphobilinogen deaminase activities. The gene which complemented this mutation was cloned to a high-copy-number plasmid, and porphobilinogen deaminase activity was restored to normal levels, but the synthesis of 5-aminolevulinate dehydratase increased 20- to 30-fold. A maxicell procedure confirmed that the gene cloned was hemB. |
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