培养基及其组成对水母雪莲悬浮培养细胞生长及黄酮形成的影响

8种不同的基本培养基对水母雪莲(Saussurea medusa)细胞生长和黄酮形成的影响不同。实验结果表明，MS培养基较有利于细胞生长和黄酮形成。从MS培养基修饰得到的MG和MP培养基比前者细胞生长量与黄酮产量分别提高32%和70%。碳源、氮源、植物激素对细胞生长及黄酮形成的影响较为明显。用HPLC对细胞培养物中两种有效黄酮(Jaceosidin和Hispidulin)作了定性定量分析。

Effects of Media on the Production of Flavonoids by Suspension Cultures of Saussurea medusa

Flavonoids were produced from cell suspension cultures of Saussurea medusa. The results of studies on eight types of culture media showed that the MS medium was the best for cell growth and fiavonoids formation, We investigated the effects of all the components of MS medium on the cell growth and fiavonoids production, and found that carbon, ni trogen and phytohormone had especially marked effects. With MG medium a modified MS medium, the yield of cell growth was 24.8 g(dwt)/L, with MP medium another modified MS mediums, the yield of fiavonoids production was 1.75 g/L. The yield of cell growth and flavonoids production in MG and MP medium were 32% and 70% higher than that in MS medium respectively.