Molecular confirmation of a Trichophyton violaceum isolate from human black-dot ringworm |
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Authors: | Okabayashi Ken Kano Rui Nakamura Yuka Ooka Shiho Kashima Masato Mizoguchi Masako Watanabe Shinichi Hasegawa Atsuhiko |
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Institution: | (1) Department of Dermatology, Teikyo University School of Medicine, 11-1, Kaga-2, Itabashi-ku, Tokyo 173-8605, Japan;(2) Department of Pathobiology, Nihon, University School of Veterinary Medicine, 1866, Kameino, Fujisawa, Kanagawa 252-8510, Japan;(3) Department of Dermatology, St. Marianna University School of Medicine, 2-16-1, Sugao, Miyamae-ku, Kawasaki, Kanagawa 216-8511, Japan |
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Abstract: | A clinical isolate from a black-dot ringworm lesion of a 28-year-old female Japanese was investigated by morphological and
biochemical analyses as well as molecular analyses. The isolate grew well onthiamine enriched agar and did not produce violetpigment,
macroconidia or microconidia on Sabouraud's dextrose agar. Approximately 620-bp genomic DNA fragments of the CHS1 gene were
amplified from Trichophyton mentagrophytes, T. rubrum, T. tonsurans and T. violaceum by polymerase chain reaction (PCR) and sequenced. The chitin synthase 1 (CHS1) nucleotide sequences of the clinical isolate
showed more than 97% similarity to that of T. violaceum and less than 96% similarity to that of T. mentagrophytes, T. rubrum and T. tonsurans. The phylogenetic analysis of their sequences revealed that the clinical isolate was genetically close to T. violaceum and distinct from T. mentagrophytes, T. rubrum and T. tonsurans. Therefore, the isolate was confirmed as T. violaceum by mycological examination and molecular analyses.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | black-dot ringworm chitin synthase gene phylogeny Trichophyton rubrum T tonsurans T violaceum |
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