Bax inserts into the mitochondrial outer membrane by different mechanisms |
| |
Authors: | Cartron Pierre-François Bellot Grégory Oliver Lisa Grandier-Vazeille Xavier Manon Stephen Vallette François M |
| |
Affiliation: | Centre de Recherche en Cancérologie Nantes-Angers, INSERM U 892, IFR 26, 9 Quai MONCOUSU, F-44035 Nantes, Cedex 01, France; Université de Nantes, Faculté de Médecine, 9 Quai Moncousu F-44035 Nantes, Cedex 01, France. |
| |
Abstract: | Bax insertion into the mitochondrial outer membrane is essential for the implementation of apoptosis. However, little is known about the first stage of Bax integration into the mitochondrial outer membrane. We have recently shown that TOM22, a mitochondrial outer membrane receptor, is important for insertion, although other reports have suggested that only mitochondrial lipids are involved in this process. Here, we show that monomers, but not dimers, of Bax require the presence of TOM22 and TOM40 to integrate into mitochondria. In addition we show that once inserted into the membrane, Bax can act as a receptor for cytosolic Bax. |
| |
Keywords: | dBax, dimeric Bax IVT, in vitro translated mBax, monomeric Bax RFP, red fluorescent protein TOM, translocase of the outer mitochondrial membrane PBR, peripheral benzodiazepine receptor VDAC, voltage dependent anionic channel |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|