Bicarbonate measurements in rat liver, brain, heart, and skeletal muscle. |
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| Authors: | M T King J L Gamble R L Veech |
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| Institution: | 1. Laboratory of Metabolism, NIAAA, Rockville, Maryland USA;2. Department of Physiology, Johns Hopkins University, School of Medicine, Baltimore, Maryland USA |
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| Abstract: | A method is described for measuring total CO2 and HCO3? in tissues rapidly frozen in liquid nitrogen. The method is a modification of the procedure of D. D. Van Slyke and J. M. Neill (1924, J. Biol. Chem.61, 523–573) for use with freeze-clamped tissue where anoxic changes have not occurred. The HCO3? content exclusive of tissue CO2 in fed rats was found to be: liver, 19.4 ± 1.0; brain, 20.2 ± 0.9; thigh, 16.2 ± 0.8; and heart, 15.4 ± 1.4 μmol/g. |
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| Keywords: | Address correspondence to M Todd King Laboratory of Metabolism NIAAA 12501 Washington Avenue Rockville Md 20852 |
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