Increase in synthesis of human monoclonal antibodies by transfected Sp2/0 myeloma mouse cell line under conditions of microgravity |
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Authors: | LJR Foster D Catzel S Atwa M Zarka SM Mahler |
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Institution: | (1) Biopolymer Research Group, University of New South Wales, Sydney, NSW, 2052, Australia;(2) School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia |
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Abstract: | Microgravity can influence cell growth and function. A transfected Sp2/0 myeloma cell line P3A2 producing a human IgG1 anti-TNF monoclonal antibody was cultivated in static culture, spinner flasks and simulated microgravity using a rotating wall vessel bioreactor. Microgravity significantly decreased cell growth (from 1.7×106 to 7.9×105 cells/ml), but facilitated the synthesis of antibodies, (1.8, 1.3 and 0.5 g of anti-TNF hmAb per 106 viable cells for cells cultivated under microgravity, in spinner flasks and static cultures, respectively). The results suggest that microgravity could be applied to improve the specific productivity of cell lines producing potentially important therapeutic proteins. |
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Keywords: | human monoclonal antibodies microgravity myeloma |
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