Effect of long-term in vitro shoot culture on somatic embryogenesis of quince leaves treated with different light qualities |
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Authors: | G Bellocchi C D'Onofrio S Morini |
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Institution: | (1) Istituto di Ricerca per le Colture Industriali, via di Corticella 133, 40128 Bologna, Italy;(2) Dipartimento di Coltivazione e Difesa delle Specie Legnose, via del Borghetto 80, 56124 Pisa, Italy |
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Abstract: | Summary The effect of long-term in vitro shoot culture on somatic embryogenesis in quince BA 29 was investigated. Three experiments were performed on leaves explanted
at about 8-mo. intervals from the same culture stock and maintained under different light qualities. Embryo production was
assessed either in terms of percentage of embryogenic leaves or number of embryos per leaf. By appropriate data processing
both these responses were linearly related to photoequilibrium in each experiment. Statistical comparisons among the three
experiments showed significant differences both in mean (computed over light qualities) and line slope values. In particular,
with increasing shoot culture age, both percentage of embryogenic leaves and number of embryos per leaf progressively increased,
while mean slope values decreased. The increase in mean values suggests a positive effect on somatic embryogenesis due to
possible tissue rejuvenation when mother cultures were cultivated in vitro for longer periods. Slope decrease over time indicated the interactions between age of the in vitro culture and photoequilibrium. Thus, embryo production at different culture ages was consistently found to be highest at high
photoequilibrium values; in contrast, if a low level of phytochrome was activated, embryogenesis in the youngest cultures
was low or absent, but increased with the progressive tissue rejuvenation arising from long-term in vitro culture. |
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Keywords: | Cydonia oblonga morphogenesis photoequilibrium rejuvenation |
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