A two step displacement and affinity chromatography purification in lactate dehydrogenase recovery from beef heart extract

Lactate dehydrogenase from beef heart extract was purified by displacement chromatography on a Tris Acryl DEAE. Chondroitin sulphate C, alginate and Eudragits were tried as displacers. Displacement, in conjunction with a subsequent affinity step, gave high purifications and yields. The potential of this operational mode as an early step in protein recovery and the use of L and S forms of Eudragit as cheap readily available and non-toxic displacers has been demonstrated in this work.