Comparison of various media and various methods for the growth and isolation of Pityrosporum pachydermatis

The pathogenic role of Pityrosporum pachydermatis in otitis externa of dogs and the related diagnostic problems are emphasized. We report results related to isolation, cultivation and identification of yeast. Agar nutritive glucosate with 1,5% of yeast extract has been showed as the best medium permitting identification in 24 hrs, associated with morfological test. Tween 80 integration (1%) to the medium permits to isolate lipolitic yeasts also.