Effects of antioxidants on calcium signal induced by cholecystokinin in mouse pancreatic acinar cells |
| |
Authors: | C Camello P J Camello J A Pariente G M Salido |
| |
Institution: | Department of Physiology, Faculty of Veterinary Sc, University of Extremadura, Cáceres, Spain. |
| |
Abstract: | Digital imaging fluorescence microscopy was used to study the effect of two antioxidants, N-acetyl-cysteine (NAC) and glutathione, on the cytosolic free calcium concentration (Ca2+]i) induced by cholecystokinin-octapeptide (CCK-8) of mouse pancreatic acinar cells. When acinar cells were preincubated with either NAC or glutathione, subsequent stimulation with CCK-8 in the presence of each antioxidant had no significant effect on the typical pattern of Ca2+]i transient evoked by the gastrointestinal hormone. However, application of NAC to acinar cells pretreated for 60 min with the same antioxidant, strongly blocked the oscillatory pattern initiated by CCK-8, inhibiting both amplitude and frequency of calcium oscillations. By contrast, glutathione had no effect on the oscillatory pattern evoked by CCK-8. The present results allow us to speculate that during Ca2+]i oscillation there is a production of oxidants that facilitate oscillations by enhancing release of calcium from internal stores. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|