Induction of tetraploidy in Buddleia globosa |
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Authors: | Rose J B Kubba J Tobutt K R |
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Institution: | (1) Horticulture Research International, East Malling, West Malling, Kent, ME19 6BJ, UK requests for offprints; Tel: +44(0)1732 843833; Fax: +44(0)1732 521557; E-mail: judy.rose@hri.ac.uk) |
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Abstract: | The aim was to produce a tetraploid form of Buddleia globosa to facilitate introgression of yellow flower colour into B. davidii, which is naturally tetraploid. Protocols were established for the micropropagation of B. globosa and tetraploid plants were obtained by application in vitro of colchicine to pre-cultured excised nodal sections. Three concentrations of colchicine were applied (0.01%, 0.05% and 0.1%
w/v) for 1, 2 or 3 days. At 0.01% tetraploids were produced only after 2 days of application. All other treatments produced
at least one tetraploid. The colchicine technique was extremely effective: of 29 lines tested, 19 were tetraploid and 5 were
mixoploid. The vegetative characteristics of these tetraploids are described and the flowering characteristics of the three
that flowered.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | Buddleia × weyeriana colchicine flow cytometry micropropagation ploidy |
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