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A self-ligation method for PCR-sequencing the telomeres of Streptomyces and Mycobacterium linear replicons |
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| Authors: | Fan Yun Dai Yumei Cheng Qiuxiang Zhang Guangjun Zhang Dongshu Fang Ping Wu Hang Bai Linquan Deng Zixin Qin Zhongjun |
| Institution: | Key laboratory of Synthetic Biology, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, People's Republic of China. |
| Abstract: | Actinomycete species from many genera often harbor linear plasmids and some contain linear chromosomes. A self-ligation and PCR-sequencing method was developed for identifying three novel telomere sequences of linear plasmids of Streptomyces and Mycobacterium. This and four previously described methods for actinomycetes telomere cloning and sequencing are discussed. |
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