Monoclonal antibodies to surface glycoconjugates in Chlamydomonas eugametos recognize strain-specific O-methyl sugars |
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Authors: | Weiger Luit Homan Henriette van Kalshoven Arend H. J. Kolk Alan Musgrave Frank Schuring Herman van den Ende |
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Affiliation: | (1) Department of Plant Physiology, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam;(2) Department of Tropical Hygiene, Royal Tropical Institute, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands |
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Abstract: | Monoclonal antibodies are described that are directed against cell surface components of the unicellular green alga Chlamydomonas eugametos. These antibodies recognize strain-specific epitopes which occur at the surface of vegetative and gametic cells. Two different groups of epitopes are distinguished that are never detectable together in one clonal cell culture. Evidence is presented showing that the antigenicity of cell surface molecules is a consequence of the presence of particular O-methylated sugars. Monoclonal antibodies reacting with one group of epitopes were studied in more detail, and immunoprecipitation and Western-blot studies showed that these epitopes can be arranged into four classes. The use of these monoclonal antibodies as strain-specific markers in light- and electron-microscopical techniques is illustrated.Abbreviations ELISA enzyme-linked immunosorbent assay - mt+/- mating type plus or minus - PAS periodic acid Schiff - Mab monoclonal antibody - PBS phosphate-buffered saline |
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Keywords: | Chlamydomonas Glycoprotein Monoclonal antibody Sugar (O-methylated) |
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