Positive effects of Taxol pretreatment on morphology, distribution and ultrastructure of mitochondria and lipid droplets in vitrification of in vitro matured porcine oocytes |
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Authors: | Xiang-Wei Fu Wen-Qing Shi Qing-Jin Zhang Xue-Ming Zhao Chang Liang Yan Yun-Peng Hou Guang-Bin Zhou Zhi-Qiang Fan Lun Suo Abuliz Wusiman Yan-Ping Wang Shi-En Zhu |
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Institution: | aCollege of Animal Science and State Key Laboratories for Agrobiotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100094, PR China;bBeijing General Station of Animal Husbandry and Veterinary Service, No. 15 Beiyuan Road, Chaoyang District, Beijing 100107, PR China |
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Abstract: | This study was designed to determine the effects of Taxol pretreatment on the morphology, distribution and ultrastructure of mitochondria and lipid droplets in vitrified porcine oocytes matured in vitro. The result showed that: (1) the rate of normal mitochondria distribution in fresh group (92.85%) was significantly higher (P < 0.05) than that in other three groups (toxicity, 72.48%; vitrification, 50.83%; Taxol + vitrification, 69.98%) and Taxol pretreatment significantly (P < 0.05) increased the ratio of normal mitochondria distribution in vitrified oocytes; (2) lipid droplets in vitrified oocytes got cracked, resulting in a great number of smaller lipid droplets (diameter <5 μm). The number of lipid droplets (5–10 μm in diameter) in vitrified oocytes pretreated with Taxol was higher (P < 0.05) than that in the oocytes without Taxol pretreatment (81.87 ± 13.63 vs. 64.27 ± 13.72); (3) both toxicity and vitrification cause the difference in the ultrastructure of mitochondria and lipid droplets. Mitochondria were well maintained in the form of typical round and ellipse shape with smooth surface and clear outline and lipid droplets existed in the form of integrity in Taxol pretreatment group.In conclusion, Taxol pretreatment has positive effects on vitrified porcine oocytes matured in vitro in terms of morphology, distribution and ultrastructure of mitochondria and lipid droplets. |
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Keywords: | Taxol Mitochondria Lipid droplets Vitrification Oocytes Porcine |
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