| 伤寒-鼠伤寒重组株Vi4072在小鼠中定居及血清学效果观察试验 |
| |
| 引用本文： | 沈玉霖,曹韫旭.伤寒-鼠伤寒重组株Vi4072在小鼠中定居及血清学效果观察试验[J].微生物学免疫学进展,1993(2):1-4. |
| |
| 作者姓名： | 沈玉霖 曹韫旭 |
| |
| 作者单位： | 卫生部兰州生物制品研究所，卫生部兰州生物制品研究所，卫生部兰州生物制品研究所，卫生部兰州生物制品研究所，卫生部兰州生物制品研究所，上海第二军医大学，上海第二军医大学，上海第二军医大学 兰州 730046，兰州 730046，兰州 730046，兰州 730046，兰州 730046，上海 200433，上海 200433，上海 200433 |
| |
| 摘 要： | 将编码Vi抗原的基因克隆到减毒的鼠伤寒沙门氏菌中组建的基因重组株Vi4072,以3×10~8CFU一次口服感染Balb/C小鼠,4天后按7,14,21,28,35,42,49,56,63,70天的间隔收集分离小鼠的集合淋巴结,肝,脾.鉴别是否有本菌出现,并检测血清和小肠匀浆液中的vi抗体.结果表明,感染后49天仍可从脾中分离到该菌;70天仍可从血清和小肠匀浆液中测出vi抗体。
|
| 关 键 词： | 鼠伤寒 沙门氏菌 Vi抗原 免疫应答 口服接种 |
Studies on the localization and serological response of the recombinant Vi 4072 in mice |
| |
| Shen Yulin et al
Lanzhou Institute of Biological Products,Lanzhou.Studies on the localization and serological response of the recombinant Vi 4072 in mice[J].Progress In Microbiology and Immunology,1993(2):1-4. |
| |
| Authors: | Shen Yulin
Lanzhou Institute of Biological Products Lanzhou |
| |
| Abstract: | The Balb/c mice were fed with the recombinant strain Vi 4072 which was const ructed by genetic recombination using a aviru-tent strain of Sal.typhimurium as a receptor, and the geae coding for Vi was transformed to it. One dose was fed about 3 × 108CFU per m use. 4 days after the feeding at an interval 7, 14, 21, 28, 35, 42, 49, 56, 63 and 70days the mouse was sacrificed.The Peyers patch. liver and spleen were collected separately for identification whether the strain fed were present.The serum andemulsion of. the small intestine were examined if there wene Vi antibodies. The results showed that the isolates were positive last for 49 days in the spleen and Vi antibodies at least 70days in the serum. |
| |
| Keywords: | Salmonella typhimurium Vi Immune response Oral immunization |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
