Isolation and characterization of an immunoenhancing glucan from alkaline extract of an edible mushroom, Lentinus squarrosulus (Mont.) Singer |
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| Authors: | Bhunia Sanjoy Kumar Dey Biswajit Maity Kankan K Patra Sukesh Mandal Soumitra Maiti Swatilekha Maiti Tapas K Sikdar Samir R Islam Syed S |
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| Affiliation: | aDepartment of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India;bDepartment of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur 721302, West Bengal, India;cDivision of Plant Biology, Bose Institute, P-1/12, C.I.T. Scheme VII M, Kolkata 700054, West Bengal, India |
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| Abstract: | A water-soluble glucan, isolated from the alkaline extract of the fruit bodies of an edible mushroom, Lentinus squarrosulus (Mont.) Singer was found to consist of (1→3,6)-linked, (1→3)-linked, (1→6)-linked, and terminal β-d-glucopyranosyl moieties in a relative proportion of approximately 1:2:1:1. This polysaccharide showed optimum activation of macrophages as well as splenocytes and thymocytes at 10 μg/mL. Structural investigation was carried out using sugar analysis, methylation analysis, periodate oxidation study, and NMR experiments (1H, 13C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of above-mentioned experiments, the structure of the repeating unit of the polysaccharide was established as: |
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| Keywords: | Lentinus squarrosulus (Mont.) Singer Polysaccharide Structure NMR spectroscopy Immuno-activation |
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