Structural characterization and study of immunoenhancing properties of heteroglycan isolated from a somatic hybrid mushroom (PfloVv1aFB) of Pleurotus florida and Volvariella volvacea |
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| Authors: | Patra Sukesh Maity Kankan K Bhunia Sanjay K Dey Biswajit Mandal Soumitra Maiti Tapas K Sikdar Samir R Islam Syed S |
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| Institution: | aDepartment of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721 102, West Bengal, India;bDepartment of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur 721 302, West Bengal, India;cDivision of Plant Biology, Bose Institute, Centenary Building, P-1/12, C.I.T Scheme VII M, Kolkata 700 054, West Bengal, India |
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| Abstract: | A water soluble polysaccharide isolated from the hot aqueous extract of the fruit bodies of the somatic hybrid mushroom (PfloVv1aFB), raised through protoplast fusion between the strains of Pleurotus florida and Volvariella volvacea was found to consist of d-glucose, d-galactose, and d-mannose in a molar ratio of nearly 4:1:1 and showed macrophage, splenocyte, and thymocyte activation. On the basis of sugar analysis, methylation analysis, periodate oxidation, and NMR studies (1H, 13C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC and HMBC), the structure of the repeating unit of the polysaccharide was established as: |
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| Keywords: | Somatic hybrid mushroom (PfloVv1aFB) Heteroglycan Structure NMR studies Immunoactivation |
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