Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O28 |
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| Authors: | Ovchinnikova Olga G Kocharova Nina A Kondakova Anna N Bialczak-Kokot Magdalena Shashkov Alexander S Knirel Yuriy A Rozalski Antoni |
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| Affiliation: | aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation;bDepartment of Immunobiology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, PL 90-237 Lodz, Poland |
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| Abstract: | An O-polysaccharide and oligosaccharides were isolated by GPC following mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O28. The O-polysaccharide was studied by sugar and methylation analyses, 1H and 13C NMR spectroscopy, including 2D ROESY and H-detected 1H,13C HSQC and HMBC experiments, and the following structure of the branched pentasaccharide repeating unit was established:This structure was confirmed by ESI MS of the isolated tridecasaccharide consisting of the lipopolysaccharide core and one O-polysaccharide repeat. The ESI mass spectrum also enabled inferring the composition of the core oligosaccharide. |
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| Keywords: | Providencia alcalifaciens O-Polysaccharide Lipopolysaccharide Bacterial polysaccharide structure Core oligosaccharide |
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