Characterization of the Integration and Modular Excision of the Integrative Conjugative Element PAISt in Streptomyces turgidiscabies Car8 |
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| Authors: | Jose C Huguet-Tapia Dawn R D Bignell Rosemary Loria |
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| Institution: | 1Department of Plant Pathology, University of Florida, Gainesville, Florida, United States of America;2Department of Biology, Memorial University of Newfoundland, St. John''s, Newfoundland and Labrador, Canada;Virginia Tech, United States of America |
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| Abstract: | PAISt is a large genomic island located in the chromosome of the plant pathogen Streptomyces turgidiscabies Car8. The island carries clustered virulence genes, transfers to other Streptomyces species, and integrates by site-specific recombination at the 8 bp palindrome TTCATGAA. The palindrome is located at the 3′ end of the bacitracin resistance gene (bacA). We demonstrate that PAISt is able to excise in modules by recombination of one internal and two flanking palindromic direct repeats. The gene intSt located at the 3( end of PAISt encodes a tyrosine recombinase. Site-specific recombination activity of intSt was tested and confirmed by heterologous expression in Streptomyces coelicolor. Comparative analysis of PAISt homologues in Streptomyces scabies 87–22 and Streptomyces acidiscabies 84–104 indicates that these islands have been fixed by sequence erosion of intSt and the recombination sites. |
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